Cultrex Mouse Laminin I, Pathclear 3400-010-02 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex Mouse Laminin I, Pathclear
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3400-010-02
Quantity1 mg
SpeciesMouse
Molecule NameLaminin-1
Brand/TrademarkR&D Systems, a Bio-Techne Brand
ApplicationsBioactivity
Format1 mg/mL at purity of =90% (SDS-Page).Storage Buffer: Dulbecco's Modified Eagle's Medium (DMEM) with 10 µg/ml gentamicin sulfate
NCBI Full Gene Namelaminin subunit alpha 1
NCBI Gene AliasesLAMA, PTBHS, S-LAM-alpha
StorageProduct is stable for a minimum of 3 months from date of shipmentwhen stored at –20°C in a manual defrost freezer. For optimal stability, store at –80°C. Avoid freeze-thaw cycles

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Morphogenesis and development of human telencephalic organoids in the absence and presence of exogenous extracellular matrixThe EMBO JournalNovember 15, 2023Catarina Martins‐Costa, Vincent A Pham, Jaydeep Sidhaye, Maria Novatchkova, Andrea Wiegers, Angela Peer, Paul Möseneder, Nina S Corsini, Jürgen A Knoblichpreparations were used: Matrigel LDEV‐Free (Corning, 354234); Cultrex Mouse Laminin I Pathclear (R&D Systems, 3400‐010‐02); and Cultrex Mouse Collagen IV (R&D Systems, 3410‐010‐02). No exogenous ECM (exECM −) At D10, EBs

(2) Stressed neuronal cells can recover from profound membrane blebbing, nuclear condensation and mitochondrial fragmentation, but not from cytochrome c releaseScientific reportsJuly 8, 2023You W, Zhou T, Knoops K, Berendschot TTJM, van Zandvoort MAMJ, Germeraad WTV, Benedikter B, Webers CAB, Reutelingsperger CPM, Gorgels TGMF.To induce differentiation, PC12 cells were dissociated and plated on PDL (P6407, sigma) and laminin (3400-010-02, R&D Systems) coated cell culture plate in low serum conditions (1% horse serum) and with 50 ng/ml of

(3) Extracellular Vesicles Potentiate Medulloblastoma Metastasis in an EMMPRIN and MMP-2 Dependent MannerCancers (Basel)May 4, 2023Hannah K. Jackson, Christine Mitoko, Franziska Linke, Donald Macarthur, Ian D. Kerr, Beth CoyleCollagen IV (Bio-Techne (Abingdon, UK) 3410-010-02)) diluted to 200 μg/mL in H2O and laminin I (Cultrex, 3400-010-02) and diluted to 100 μg/μL in serum-free media. This setting is thought to resemble the in vivo situation

(4) ZCCHC17 modulates neuronal RNA splicing and supports cognitive resilience in Alzheimer’s diseasebioRxivMarch 26, 2023Anne Marie W. Bartosch, Elliot H. H. Youth, Shania Hansen, Maria E. Kaufman, Harrison Xiao, So Yeon Koo, Archana Ashok, Sharanya Sivakumar, Rajesh K. Soni, Logan C. Dumitrescu, et al.embryoid bodies, followed by plating on polyornithine (10 ug/mL, Sigma P4957)/laminin (10 ug/mL, R&D Systems 3400-010-02) and subsequent manual rosette selection and expansion, as described previously (Topol et al., 2015;

(5) Generation of Schwann cell derived melanocytes from hPSCs identifies pro-metastatic factors in melanomabioRxiv : the preprint server for biologyMarch 7, 2023Samuel RM, Navickas A, Maynard A, Gaylord EA, Garcia K, Bhat S, Majd H, Richter MN, Elder N, Le D, et al.

(6) Drug Resistance in Medulloblastoma Is Driven by YB-1, ABCB1 and a Seven-Gene Drug SignatureCancersFebruary 8, 2023Taylor L, Wade PK, Johnson JEC, Aldighieri M, Morlando S, Di Leva G, Kerr ID, Coyle B.Cultrex® mouse collagen IV (Bio-Techne (Abingdon, UK) 3410-010-02). Following 24 h, 50 µL of 100 µg/mL Laminin I (Cultrex; 3400-010-02) was added to each coated insert, which was partially dried for 1 h. 1 × 105 viable cells were then

(7) In vitro myelination using explant culture of dorsal root ganglia: An efficient tool for analyzing peripheral nerve differentiation and disease modelingPloS oneJanuary 1, 2023Numata-Uematasu Y, Wakatsuki S, Kobayashi-Ujiie Y, Sakai K, Ichinohe N, Araki T.

(8) Neuroprotection of Rodent and Human Retinal Ganglion Cells In Vitro/Ex Vivo by the Hybrid Small Molecule SA-2CellsNovember 23, 2022Pham JH, Johnson GA, Rangan RS, Amankwa CE, Acharya S, Stankowska DL.

(9) Differentiation and on axon-guidance chip culture of human pluripotent stem cell-derived peripheral cholinergic neurons for airway neurobiology studiesFrontiers in PharmacologyOctober 28, 2022P. A. Goldsteen, A. M. Sabogal Guaqueta, P. P. M. F. A. Mulder, I. S. T. Bos, M. Eggens, L. Van der Koog, J. T. Soeiro, A. J. Halayko, K. Mathwig, L. E. M. Kistemaker, et al.Sigma-Aldrich, P4538), 2 μg/ml fibronectin (FB, Thermo Fisher, 33016015), and 2 μg/ml laminin (LM, R&D systems, 3400-010-02). In the first stage of airway cholinergic neuron induction (day 16–30), the medium was changed three

(10) CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entryiScienceOctober 21, 2022Elena-Sofia Heinl, Sebastian Lorenz, Barbara Schmidt, Nouf Nasser M Laqtom, Joseph R. Mazzulli, Laetitia Francelle, Timothy W. Yu, Benjamin Greenberg, Stephan Storch, Ines Tegtmeier, et al.(peqGold)",VWR International (US),Cat#PEQL35-1030AL Cultrex Mouse Laminin I,R&D Systems/Bio Techne GmbH,Cat # 3400-010-02 Bovine Fibronectin,PromoCell,Cat#C-43060 Collagen I from calf-skin,Sigma-Aldrich (DE),Cat#C8919 Cultrex Mouse Collagen IV

(11) Substantial somatic genomic variation and selection for BCOR mutations in human induced pluripotent stem cellsNature geneticsSeptember 1, 2022Rouhani FJ, Zou X, Danecek P, Badja C, Amarante TD, Koh G, Wu Q, Memari Y, Durbin R, Martincorena I, et al.(ThermoFisher Scientific, A3890401, lot 881772E) and 15 µg ml−1 Cultrex mLaminin I Pathclear (Biotechne, 3400-010-02; Lot: 1594368). The NIM was switched to neuron differentiation medium (NDM) containing BrainPhys Neural

(12) Protocol to assess the effect of disease-driving variants on mouse brain morphology and primary hippocampal neuronsSTAR protocolsJune 17, 2022de Prisco N, Chemiakine A, Lee W, Botta S, Gennarino VA.,Thermo Fisher Scientific,Cat# 21103049 Poly-D-Lysine Solution,Thermo Fisher Scientific,Cat# A003E Laminin I,R&D systems,Cat# 3400-010-02 TrypLE Express Enzyme 1×,Thermo Fisher Scientific,Cat# 12604013 5-Fluoro-2′-deoxyuridine,MilliporeSigma,Cat#

(13) Immune-mediated neurodegenerative trait provoked by multimodal derepression of long-interspersed nuclear element-1iScienceMay 20, 2022Takahashi F, Zhang C, Hohjoh H, Raveney B, Yamamura T, Hayashi N, Oki S.,R&D systems,Cat#404-ML-010/CF Poly-L-lysine hydrobromide,Sigma-Aldrich,Cat#P2636-100MG Laminin,R&D systems,Cat#3400-010-02 TNT™ Quick Coupled Transcription/Translation System,Promega,Cat#PR-L1171 Kynurenine ELISA high sensitive

(14) Single Cell Analysis of Reversibility of the Cell Death Program in Ethanol-Treated Neuronal PC12 CellsInternational journal of molecular sciencesFebruary 28, 2022You W, Berendschot TTJM, Knoops K, van Zandvoort MAMJ, Webers CAB, Reutelingsperger CPM, Gorgels TGMF.were dissociated and plated on PDL (50 μg/mL; P6407, sigma, Darmstadt, Germany) and laminin (10 μg/mL; 3400-010-02, R&D Systems, Minneapolis, MN, USA) coated cell culture plate in low serum conditions (1% horse serum,

(15) Phosphorylated WNK kinase networks in recoded bacteria recapitulate physiological functionCell reportsJuly 20, 2021Schiapparelli P, Pirman NL, Mohler K, Miranda-Herrera PA, Zarco N, Kilic O, Miller C, Shah SR, Rogulina S, Hungerford W, et al.

(16) Efficient Derivation of Excitatory and Inhibitory Neurons from Human Pluripotent Stem Cells Stably Expressing Direct Reprogramming FactorsCurr ProtocJune 1, 2021Song S, Ashok A, Williams D, Kaufman M, Duff K, Sproul A.human NT3 (R&D Systems, cat. no. 267-N3; see recipe) Cultrex Mouse Laminin I (R&D Systems, cat. no. 3400–010-02) 1 mg/mL Doxycycline Hyclate (Sigma-Aldrich, cat. no. D9891; see recipe) BrainPhys Neuronal Medium N2-A & SM1 Kit

(17) Dissecting the impact of regional identity and the oncogenic role of human-specific NOTCH2NL in an hESC model of H3.3G34R-mutant gliomaCell stem cellMay 6, 2021Funato K, Smith RC, Saito Y, Tabar V.12-541 Poly-L-Ornithine hydrobromide,Sigma-Aldrich,Cat# P3655 Cultrex mLaminin I,R&D Systems,Cat# 3400-010-02 Fibronectin,ThermoFisher,Cat# 356008 Recombinant Human Vitronectin truncated,ThermoFisher,Cat# A14700 Recombinant

(18) Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01Cell stem cellFebruary 4, 2021Piao J, Zabierowski S, Dubose BN, Hill EJ, Navare M, Claros N, Rosen S, Ramnarine K, Horn C, Fredrickson C, et al.

(19) A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and OrganoidsCell stem cellJuly 2, 2020Yang L, Han Y, Nilsson-Payant BE, Gupta V, Wang P, Duan X, Tang X, Zhu J, Zhao Z, Jaffré F, et al.

(20) Mechanisms of hyperexcitability in Alzheimer's disease hiPSC-derived neurons and cerebral organoids vs isogenic controlseLifeNovember 29, 2019Ghatak S, Dolatabadi N, Trudler D, Zhang X, Wu Y, Mohata M, Ambasudhan R, Talantova M, Lipton SA.

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Cultrex Mouse Laminin I, Pathclear from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Cultrex Mouse Laminin I, Pathclear from R&D Systems, a Bio-Techne Brand

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